Two officials at the Vatican Bank are being investigated as part of a money-laundering inquiry, reports say.
Rome prosecutors are investigating allegations that money-laundering laws have been broken, judicial sources are quoted as saying by news agencies.
The Vatican Bank, known officially as the Institute for Religious Works, manages accounts for Roman Catholic orders.
There has been no comment from the Vatican on the reports.
The Vatican Bank was last mired in scandal in 1982 when its governor Archbishop Paul Marcinkus was indicted over his involvement with the $3.5bn (£2.25bn) collapse of what was then Italy's largest private bank, Banco Ambrosiano.
The fallout from that scandal took a darker turn when two of its top executives, including its chairman, Roberto Calvi, were murdered.
Calvi, known as God's Banker because of his close ties to the Vatican, was found hanged beneath Blackfriars' Bridge in London. The murder bore the hallmarks of a ritual killing.
BBC News